Beate Fast Krakstad

• Lien, Anne; Palmer, Helen; Larsen, Jorunn; Svendsen, Monica Lian; Bomark, Nils-Erik & Krakstad, Beate Fast [Show all 7 contributors for this article] (2026). Building a Scientific Skill Set: A Design-Based Research Approach to Developing a Tool for Science Education and Beyond | Journal of the International Society for Teacher Education. Journal of the International Society for Teacher Education. ISSN 1029-5968. 30(1), p. 5–22. doi: 10.26522/jiste.v30i1.5268. Full text in Research Archive Show summary

  Life skills like problem-solving, communication, collaboration, and creative and critical thinking are closely tied to scientific practices in science education. However, there is ongoing debate about how to define and operationalize scientific practices for implementation in schools and teacher education. In Norway, these practices are a core element of the science curriculum, but their definition remains vague. To address this, we used a design-based research approach to develop a skills-based tool for teaching and learning scientific practices in schools and teacher education. The tool highlights twelve interrelated skills that support students’ engagement in scientific practices and their reflection on scientific processes. We describe the process of designing and developing the teaching tool, our design analysis of why and how the tool evolved, and three design principles to inform the development of a skills-based teaching tool to practice science. These principles highlight three critical features of the tool: it is dynamic and flexible, initiates active learning, and provides support for teachers.

• Ardawatia, Vandana; Masià-Balagué, Miriam; Krakstad, Beate Fast; Johansson, Bente Berg; Spriet, Endy & Lewis, Aurelia Eva [Show all 8 contributors for this article] (2011). Gα(12) binds to the N-terminal regulatory domain of p120(ctn), and downregulates p120(ctn) tyrosine phosphorylation induced by Src family kinases via a RhoA independent mechanism. Experimental Cell Research. ISSN 0014-4827. 317(3), p. 293–306. doi: 10.1016/j.yexcr.2010.10.017. Full text in Research Archive
• Ardawatia, Vandana; Masià-Balagué, Miriam; Krakstad, Beate Fast; Johansson, Bente Berg; Kreitzburg, Kelly M. & Spriet, Endy [Show all 9 contributors for this article] (2011). G alpha(12) binds to the N-terminal regulatory domain of p120(ctn), and downregulates p120(ctn) tyrosine phosphorylation induced by Src family kinases via a RhoA independent mechanism. Experimental Cell Research. ISSN 0014-4827. 317(3), p. 293–306. doi: 10.1016/j.yexcr.2010.10.017. Full text in Research Archive
• Minsaas, Laura; Planaguma, Jesus; Madziva, Michael; Krakstad, Beate Fast; Masià-Balagué, Miriam & Katz, Arieh A. [Show all 7 contributors for this article] (2010). Filamin A binds to CCR2B and regulates its internalization. PLOS ONE. 5(8). doi: 10.1371/journal.pone.0012212. Full text in Research Archive Show summary

  The chemokine (C-C motif) receptor 2B (CCR2B) is one of the two isoforms of the receptor for monocyte chemoattractant protein-1 (CCL2), the major chemoattractant for monocytes, involved in an array of chronic inflammatory diseases. Employing the yeast two-hybrid system, we identified the actin-binding protein filamin A (FLNa) as a protein that associates with the carboxyl-terminal tail of CCR2B. Co-immunoprecipitation experiments and in vitro pull down assays demonstrated that FLNa binds constitutively to CCR2B. The colocalization of endogenous CCR2B and filamin A was detected at the surface and in internalized vesicles of THP-1 cells. In addition, CCR2B and FLNa were colocalized in lamellipodia structures of CCR2B-expressing A7 cells. Expression of the receptor in filamin-deficient M2 cells together with siRNA experiments knocking down FLNa in HEK293 cells, demonstrated that lack of FLNa delays the internalization of the receptor. Furthermore, depletion of FLNa in THP-1 monocytes by RNA interference reduced the migration of cells in response to MCP-1. Therefore, FLNa emerges as an important protein for controlling the internalization and spatial localization of the CCR2B receptor in different dynamic membrane structures.

• Jiménez-Sainz, M. Carmen; Murga, Cristina; Kavelaars, Annemieke; Jurado-Pueyo, María; Krakstad, Beate Fast & Heijnen, Cobi J. [Show all 8 contributors for this article] (2006). G protein-coupled receptor kinase 2 negatively regulates chemokine signaling at a level downstream from G protein subunits. Molecular Biology of the Cell. ISSN 1059-1524. 17(1), p. 25–31. doi: 10.1091/mbc.E05-05-0399. Full text in Research Archive
• Krakstad, Beate Fast; Ardawatia, VV & Aragay, Anna M. (2004). A role for G alpha(12)/G alpha(13) in p120(ctn) regulation. Proceedings of the National Academy of Sciences of the United States of America (PNAS). ISSN 0027-8424. 101(28), p. 10314–10319. Full text in Research Archive

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• Krakstad, Beate Fast (2004). Involvement of G protein Pathways in Cell Adhesion and Migration. Universitetet i Bergen. Full text in Research Archive

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